16, 9677–86. The luminescence signal from these reporter vectors is used as an indicator of the activity of any gene/DNA element fused to the reporter. Vector Pgemt Easy, supplied by Promega, used in various techniques. Promega’s pGEM-T vector systems are very dependable and versatile tools to routinely clone fragments of DNA into reliable plasmids. Clark, J.M. • Construction of unidirectional nested deletions with the Erase-a-Base® System. Get cells out of freezer, thaw on ice for 5 minutes 2. Bioz Stars score: 99/100, based on 161 PubMed citations. 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase 2. • In vitro transcription from dual-opposed promoters. Die von Ihnen gewünschte Seite kann nicht gefunden werden, oder ist momentan nicht erreichbar. Robles, J. and Doers, M. (1994) Promega Notes 45, 19–20. Video Protocols. (1988) Nucl. The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Complete Protocol. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 177 PubMed citations. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 161 PubMed citations. “Reporter Gene Assay Kits Market Scenario 2020-2027:. You are viewing: E1910 Change Configuration. (1994) In: PCR, BIOS Scientific Publishers, Ltd., Oxford, UK, 13. 1/21 Apoptosis Assays ProductSize Cat.# Caspase-Glo® 3/7 Assay System 2.5ml G8090 Caspase-Glo® 3/7 3D Assay System 10ml G8981 RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay 100 assays JA1011 Additional … Protein expression vectors are basic tools for production of proteins. The product portfolio includes RNA purification solutions for all sample throughput needs, from manual purification to high-throughput automated systems. 3 µl Fresh PCR product b. Die Promega … Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3600; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). ベクターマップ＆シークエンス Item Part # Size Available Separately; Luciferase Assay Substrate. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 16 PubMed citations. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. Add to the reaction mixture: a. Cloning - Promega pGEM-T Easy kit Ligation 1. The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation reactions. A protocol for isolation of genomic DNA from white blood cells, tissue culture cells, animal and plant tissue, yeast, Gram-positive and Gram-negative bacteria. Technical Manual pGEM®-T and pGEM®-T Easy Vector Systems カタログカタログカタログ番号カタログ番号番号番号 A1360, A1380, A3600, A3610 www.promega.co.jp 注意注意注意：注意：：： この日本語マニュアルは製品に添付される英文マニュアルを翻訳したもの ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 572 PubMed citations. Promega RNA purification products extract high-quality total RNA, mRNA or miRNA from many sample types, including blood, cells, tissues, FFPE tissue and total RNA preparations. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid. www.promega.com Part# TM042 Printed in USA. Promega AG wurde in der Schweiz 1991 unter dem Namen Catalys AG gegründet. The pGEM-T and pGEM-T Easy Systems now allow you to perform ligation reactions in as little as one hour. Pgemt Easy Vector, supplied by Promega, used in various techniques. These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). Pgemt Promega Polylinker, supplied by Promega, used in various techniques. The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. Pgemt Vector, supplied by Promega, used in various techniques. e-mail: office@promega.cc homepage: www.promega.cc Filmenbuchgericht: Feldkirch Filmenbuch: FN 336296d UST-IdNr. Designed to work seamlessly with Promega assays, GloMax® microplate readers and luminometers come preloaded with protocols that allow you to run assays at the touch of a button. Related Posts. プロメガ（Promega Corporation）はライフサイエンスの分野に生化学および分子生物学に関連する革新的で価値の高い製品を世界の研究者にお届けするリーディングカンパニーです。 Many Promega Vectors are reporter vectors containing various combinations of luciferase genes (firefly, Renilla, NanoLuc luciferase) with other genetic elements. GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. • Production of ssDNA. Incubate at room temperature for 1 hour or overnight at 4°C 3. Purified DNA is suitable for amplifications, restriction enzyme digestion and membrane hybridizations (e.g., Southern and dot/slot blots). Quick Protocols. Seite nicht gefunden. Schweden. The pGEM-T Easy vector has EcoRI restriction sites surrounding the proposed insert site, whereas the pGEM-T vector does not. (2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. Protocols. Theoretically, smaller inserts will be cloned more efficiently than longer inserts. The Global Reporter Gene Assay Kits market exhibits comprehensive information that is a valuable source of insightful data for business strategists during the decade 2014-2027. Read More About Sensitivity. DA: 59 PA: 43 MOZ Rank: 15. ATU65297145 ARA -Nummer: 17418 PROMEGA Handelsgesellschaft m.b.H Treietstraße 1 A-6833 Klaus Cloning with pGEM®-T Vectors: Ligation; January 8, 2020; A Quick Method for A Tailing PCR Products; July 8, 2019; T-Vector Cloning: Questions, Answers and Tips ; November 12, 2019; The following two tabs change content below. Eukaryotische Expressionssysteme Analyse der Top-Unternehmen 2021 – Thermo Fisher, Agilent, New England Biolabs, Sigma Aldrich, Promega apexresearch März 9, 2021 Nischenmarktforschungsbericht 2021 über Eukaryotische Expressionssysteme Markt mit Branchenwachstumsanalyse, Wettbewerb, Trends und Prognose bis 2026. Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3610; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Use the DNA molecular weight markers for both conventional and pulsed field gel electrophoresis applications. Bioz Stars score: 97/100, based on 2 PubMed citations. and Graham, A. • Construction of unidirectional nested deletions with the Erase-a-Base® System. Promega AG beschäftigt 16 Mitarbeiter. Luminometer and Microplate Reader Selection Guide. Store on ice until needed Transformation 1. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual PDF (548 KB) – English. 製品マニュアル（日本語） DH5α使用説明書. Acids Res. Instructions for Use of Product(s) M7121: GoTaq Green Master Mix, M7122, M7123 Literature # 9PIM712. • Blue/white screening for recombinants. プロトコル クイックプロトコル (pGEM-T Vectors) 製品マニュアル. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 2 PubMed citations. Bioz Stars score: 99/100, based on 177 PubMed citations. Bioz Stars score: 99/100, based on 16 PubMed citations. What's in the box? Page 4 Revised 5/07 GGAGA GCTCC CAACG CGTTG GATGC ATAGC TTGAG TATTC TATAG … Dual-Luciferase Reporter Assay and Dual-Luciferase Reporter 1000 Assay System Quick Protocol FB024 PDF (73 KB) – English. Newton, C.R. On the basis of historical data, Reporter Gene Assay Kits market report provides key segments and their sub-segments, revenue and demand & supply data. Choose from a range of DNA, RNA and protein molecular weight markers. Bioz Stars score: 97/100, based on 572 PubMed citations. • Production of ssDNA. Promega Technical Services Scientists are here to assist you in troubleshooting your experiments at any time. pGEMt_vector - Free download as PDF File (.pdf), Text File (.txt) or read online for free. The pGEM-T and pGEM-T Easy plasmid vectors are essentially the same but with one important difference. • In vitro transcription from dual-opposed promoters. Falls Sie über einen defekten Link hierher gekommen sind, schreiben Sie bitte eine E-Mail an den Webmaster (webmaster@promega.cc).Falls es … E151A: 1 × 1 vial: Passive Lysis Buffer, 5X. Specifications. Contact Technical Services. Pgemt, supplied by Promega, used in various techniques. Theoretically, smaller inserts will be cloned more efficiently than longer inserts. Instructions for Use of Product(s) E1910, E1960. Als Tochtergesellschaft von Promega Corp. bietet die Schweizer Niederlassung in Dübendorf zusätzlich und komplementär zu Produkten von Promega Corp. auch Labor- und Analysegeräte sowie Verbrauchsmaterialien der Firmen Serva GmbH an. • Blue/white screening for recombinants. Literature # TM040.

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