Proszę sprawdzić połączenie internetowe i spróbować rejestracji ponownie. pGEM®-T Vector Map and Sequence The pGEM®-T Vector is derived from the pGEM®-5Zf (+) Vector (GenBank® Accession No. Figure 1. The pGEM®-T Easy Vector multiple cloning region is flanked by recognition sites for the restriction enzymes EcoRI, BstZI and NotI, thus providing three single-enzyme digestions for release of the insert. The pGEM®-T Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. The pGEM®-T Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Vector System I components. Specifications. Our website uses functional cookies that do not collect any personal information or track your browsing activity. In the pGEM®-T Vector, T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. w10.0.13 | c1.0.0.2. Wysokowydajna polimeraza DNA Taq do codziennych potrzeb PCR. PLos ONE, Badania serologiczne SARS-CoV-2 i testy PCR, Badania w kierunku wirusów i rozwój szczepionek, Rapid Ligation for the pGEM®-T and pGEM®-T Easy Vector Systems, Comparing Cloning Efficiency of the pGEM®-T and pGEM®-T Easy Vectors to the TOPO TA Cloning® Vectors, Shorten the Ligation Time for the pGEM®-T Vector Systems, TRE5-A retrotransposition profiling reveals putative RNA polymerase III transcription complex binding sites on the, Polityka prywatności i przetwarzania danych, Promega GmbH General Terms and Conditions of Business, Insert excision with a BstZI single digest, Ligation can be completed in 1 hour at room temperature, Available with or without competent cells. Wysokowydajna polimeraza Taq z niezawierającymi Mg buforami reakcyjnymi. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. Następnie należy skontaktować się z Działem Obsługi Klienta w celu odblokowania konta. The pGEM ®-T and pGEM ®-T Easy Vector Systems are convenient systems for the cloning of PCR products. Proszę spróbować ponownie lub skontaktować się z Działem Obsługi Klienta. Feature Options. Gotowa do użycia zoptymalizowana mieszanina Master Mix do składania PCR w temperaturze pokojowej. We provide medical information and facilitate research collaborations. Wystąpił błąd weryfikacji adresu e-mail. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The vector allows preparation of single-stranded DNA due to its f1 Origin of Replication. Gratulacje! pGEM T and pGEM T Easy Vector Systems FB033 PDF (202 KB) – English. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual, pGEM T and pGEM T Easy Vector Systems FB033, 2017 The coding sequence was inserted by TA cloning. This addition enables the ‘easy’ restriction of the plasmid for routine cloning applications, hence the name. + Sequence information. pGEM®-T Vector Map and Sequence The pGEM®-T Vector is derived from the pGEM®-5Zf (+) Vector (GenBank® Accession No. TOP10, DH5α and TOP10F´, JM109. Regarding the pGEM-T vector I agree with Syed, you can insert the PCR fragment via T-A cloning. The incubation period may be extended to increase the number of colonies after transformation. Specifications. Alternatively, a double digestion may be used to release the insert from the vector. pGEM T and pGEM T Easy Vector Systems FB033 PDF (202 KB) – English. Spróbuj ponownie lub skontaktuj się z Obsługą Klienta. 迅速なライゲーションバッファー添付によるキットの改良. When you select your country, you agree that we can place these functional cookies on your device. This product is available through the Promega Helix onsite stocking program. PCR cloning system for expression in mammalian cells. X65308). I, pGEM-T Easy with a cloned genomic fragment comprising TcADK4 , ISs (solid bold lines) and flanking coding sequences (light grey boxes). Protocolos. The pGEM-T Easy vector has EcoRI restriction sites surrounding the proposed insert site, whereas the pGEM-T vector does not. ベクターマップ＆シークエンス. Your password reset link has expired. If initial experiments with your PCR product are suboptimal, ratio optimization may be necessary. The coding sequence was inserted by TA cloning. pGEM-T Vector Information Description The pGEM-T vector is a high-efficiency TA cloning vector which contains multiple cloning sites as shown below. All Rights Reserved. The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. SampleTextSampleText。:victory:pGEM-T_easy_vector_sequence质粒序列.docxpGEM-T_easy_vector质粒序列.txtLasteditedbysilicareon2012-10-18at17:39] XX CC pGEM-T has dT, which improves efficiency of ligation of PCR product. Trademarks The promoter and multiple cloning sequence of the pGEM®-T (Panel A) and pGEM®-T Easy (Panel B) Vectors.The top strand of the sequence shown corresponds to the RNA synthesized by T7 RNA Polymerase.The bottom strand corresponds to the RNA synthe-sized by SP6 RNA Polymerase. The pGEM®-T Vector was created by linearizing the pGEM®-5Zf (+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. pGEM-T vector backbone. Podaj nazwę użytkownika, aby otrzymać link do zresetowania hasła. Procedure: 1. The pGEM-T vector is 3.0kb in size and contains the ampicillin resistance gene for selection. Ratios from 3:1 to 1:3 provide good initial parameters. Nie można otworzyć konto bez weryfikacji adresu e-mail. pGEM®-T Easy Vector Systemは、従来のpGEM®-T Vector Systemの機能に加え、マルチクローニングサイトの両端にEcoRIとNotIサイトが加えられました。そのため、1種類（NotI、EcoRIあるいはBstZI）の制限酵素を用いるだけで、クローニング後のインサートDNAを簡単に切り出すことがきます。 Are there any tools that can assist with primer design for DNA sequencing? The pGEM-T vector is a high-efficiency TA cloning vector which contains multiple cloning sites as shown below. Podany e-mail posiada już istniejące konto. The pGEM ®-T and pGEM ®-T Easy Vector Systems include a 2X Rapid Ligation Buffer for ligation of PCR products. Your commerce experience may be limited. The pGEM ®-T and pGEM -T Easy Vector Systems have been optimized using a 1:1 molar ratio of the Control Insert DNA to the vectors. The provided 2X Rapid Ligation Buffer allows reactions to be completed in 1 hour at room temperature. The pGEM is a control template that can be used to isolate issues with sample quality, thermal cycler, kit or sequencing reaction purification. The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. a. PCR cloning vectors with 3 options for insert excision. pGEM®-T Vector Map and Sequence The pGEM®-T Vector is derived from the pGEM®-5Zf (+) Vector (GenBank® Accession No. クイックプロトコル (pGEM-T Vectors) 製品マニュアル. Usage Suggestion：The ORF cDNA sequence can be amplified by PCR with M13-47 and RV-M primers. Determine the volume of PCR product to add to the ligation. Please update your browser to Internet Explorer 11 or above. If initial experiments with your PCR product are suboptimal, ratio optimization may be necessary. A3600. Nie zweryfikowano podanego adresu e-mail. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual PDF (548 KB) – English. The pGEM®-T Vector was created by linearizing the pGEM®-5Zf (+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The parent vector is linearized at the position indicated by * in this pGEM®-T Easy Vector Sequence and a "T" is added at each end. Weryfikacja adresu e-mail jest niezbędna do utworzenia konta na promega.com. Video Protocols. The pGEM®-T Vector was created by linearizing the pGEM®-5Zf (+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. X65308). Reactions using this buffer may be incubated for 1 hour at room temperature. For clarity equivalent sequences in both constructs are only shown for pL4-GA Neo. The incubation period may be extended to increase the number of colonies after transformation. Aby chronić Twoją prywatność, konto zostanie zablokowane po 6 nieudanych próbach. REQUIRED MATERIALS PGEM-T Easy plasmid (Kit ordered from Fisher PR-1380) 2x rapid ligation buffer T4 DNA Ligase enzyme **Note a few ingredients to the Ligation reaction are NOT on your desk due to the very small volumes needed. Spróbuj ponownie lub skontaktuj się z Obsługą Klienta. The pGEM ® -T and pGEM ® -T Easy Vector Systems are convenient systems for the cloning of PCR products. Aby chronić Twoją prywatność, Twoje konto zostało zablokowane po 6 nieudanych próbach zalogowania się. Login / Register Order Menu. + Datasheet. + Compare & Order pGEM-T vector backbone products + TOP customer support. Especificaciones. Quick Protocols. Legal and Trademarks Sprawdź swoją pocztę e-mail, aby potwierdzić adres e-mail. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by Blue/White Screening on indicator plates. Twoje konto zostało utworzone. Wystąpił błąd podczas utwrozenia konta. However, ratios of 8:1 to 1:8 have been used successfully. Both the pGEM®-T and pGEM -T Easy Vector contain multiple restriction sites within the multiple cloning region. The vectors are prepared by cutting the pGEM ®-5Zf(+) and pGEM ®-T Easy Vectors, respectively, with EcoR V and adding a 3´ terminal thymidine to both ends. Let's find the product that meets your needs. The pGEM ®-T and pGEM -T Easy Vector Systems have been optimized using a 1:1 molar ratio of the Control Insert DNA to the vectors. Protocols. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt). Quick Protocols. pGEM®-T Easy, pGEM-T Easy: Analyze: Sequence: Plasmid Type: Bacterial Expression: Expression Level: High: Cloning Method: Unknown: Size: 3015: 5' Sequencing 1 Primer: T7, SP6, M13Fwd or M13Rev: Bacterial Resistance: Ampicillin: Notes: The only difference between pGEM-T and pGEM-T Easy is in the multiple cloning site (MCS). Please request another reset link. Wystąpił błąd w czasie tworzenia konta. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual PDF (548 KB) – English. The pGEM®-T Vector is ready to use in ligation reactions, prepared by cutting the pGEM®-5Zf(+) Vector with EcoRV and adding a 3´ terminal thymidine to both ends. Protocolos en Vídeo. PROD | u7.5.14. Protocolos Rápidos. Proszę skontaktować się z Działem Obsługi Klienta, aby odblokować konto. If initial experiments with your PCR product are suboptimal, ratio optimization may be necessary. Video Protocols. Read 3 answers by scientists to the question asked by Muh.Chaeril Ikramullah on Mar 3, 2021 There was an issue logging into your account. We've detected that you are using an older version of Internet Explorer. Polityka prywatności i przetwarzania danych pGEM®-T Vector Map and Sequence The pGEM®-T Vector is derived from the pGEM®-5Zf (+) Vector (GenBank® Accession No. Complete Protocol. Benefit from the greatest possible flexibility in the choice of handling and managing your sequencing primers. The pGEM control and M13 primer provided in the kit should be used for troubleshooting purposes. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt). Primer3 is a great tool to pick your primers from a particular sequence. The concentration of PCR product Dziękujemy za potwierdzenie adresu e-mail. © 2007-2021 Sino Biological Inc. All rights reserved, Common Cytokine Receptor Signaling Pathway. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt). https://www.snapgene.com/.../?set=basic_cloning_vectors&plasmid=pGEM-T A resource designed for scientists just embarking on their career, focusing on fundamental technologies and techniques. The multiple cloning site is flanked by recognition sites for the restriction enzyme BstZI, allowing release of the insert by a single-enzyme digestion. Promega GmbH General Terms and Conditions of Business. The parent vector is linearized at the position indicated by * in this pGEM®-T Easy Vector Sequence and a "T" is added at each end. Protocols. However, ratios of 8:1 to 1:8 have been used successfully. pGEM-T easy plasmid DNA (500 ng, Promega, Madison, WI, USA) was then added and incubated for 1 h at 37 °C. What do you mean by " if you are going for expression from that gene then try to avoid pGEM-T easy vector because later these overhang can cause problem in expression level." パフォーマンス. Please try again or contact Customer Service. The pGEM®-T Vector is derived from the pGEM®-5Zf(+) Vector (GenBank® Accession No. 製品マニュアル（日本語） DH5α使用説明書. See Protocol for detailed storage recommendations. Wystąpił błąd w czasie zmiany hasła. Stay notified of Promega events, products and news. pGEM T and pGEM T Easy Vector Systems FB033 PDF (202 KB) – English. The pGEM-T vector is 3.0kb in size and contains the ampicillin resistance gene for selection. Proszę spróbować ponownie lub skontaktować się z Obsługą Klienta. EVOcards. Most commercially available competent cells are appropriate for the plasmid, e.g. The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt). pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual PDF (548 KB) – English. X65308). The position of the T is indicated by * in the pGEM®-T Vector Sequence (.txt). © 2021 Promega Corporation. Wysokowydajna polimeraza DNA Taq w gotowej do użycia mieszaninie Master Mix. ベクターのT突出末端の安定性. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. Ratios from 3:1 to 1:3 provide good initial parameters. Complete Protocol. Skontaktuj się z najbliższym przedstawicielem naukowym, Catalog number selected: Complete Protocol. CC NM (pGEM-T) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (phagemid) CC SP (Promega) CC HO (E.coli) CC CP () CC FN (cloning)(transcription) CC SE (color blue/white) CC PA (pGEM-5Zf+) CC BR () CC OF () CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. pGEM-5Zf+ 3003bp FT -> pGEM-T … II, TGRVs produced by replacement of a fragment of TcADK4 with the SMs of p Tc R-HG Hyg - and p Tc R-GA Neo -. E-mail weryfikujący został wysłany na adres podany podczas rejestracji. E-mail z linkiem do zresetowania hasła został wysłany na adres podany podczas rejestracji. The parent vector is linearized at the position indicated by * in this pGEM®-T Easy Vector Sequence and a "T" is added at each end. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for ligation of PCR products with A overhangs. Wysłaliśmy na podany adres e-mail do weryfikacji. The pGEM ®-T and pGEM -T Easy Vector Systems have been optimized using a 1:1 molar ratio of the Control Insert DNA to the vectors. Your professor will come around with the PGEM-T Easy Vector and T4 DNA ligase. The pGEM®-T Vector was created by linearizing the pGEM®-5Zf(+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. .. Nicking of DNA was evaluated by ethidium bromide staining after electrophoresis separation in 0.8% agarose gels [ , ]. We offer numerous convenient solutions to meet your lab's needs. X65308). The pGEM®-T Vector was created by linearizing the pGEM®-5Zf (+) Vector with EcoRV at base 51 and adding a T to both 3´-ends. The vectors are prepared by cutting the pGEM ® -5Zf (+) and pGEM ® -T Easy Vectors, respectively, with EcoR V and adding a 3´ terminal thymidine to both ends. However, ratios of 8:1 to 1:8 have been used successfully. X65308). pGEM-T Easy Vector: 3016 bp 1 1000 2000 3000 3016 ApaI (14) AatII (20) NcoI (37) SacII (49) SpeI (65) PstI (89) SalI (91) NdeI (98) SacI (110) M13_reverse_primer Sp6_primer M13_pUC_rev_primer lac_promoter ORF frame 3 Ampicillin AmpR_promoter f1_origin lacZ_a M13_pUC_fwd_primer M13_forward20_primer.

Icône Religieuse Ancienne, Dessin Noix De Coco, Matelas Epeda Nymphe 180x200, 2 Rue Ruhmkorff 75017 Paris, Zone3 Advance Femme, Liberté D'expression états-unis Date, Toxic Skull Drawing, Service Urbanisme De Saint-denis, Métro Lausanne Tarif,